Going forward this is something that's certainly worth adding to the iso plugin. There is a system that selects the appropriate animation automatically, but right now there are only Idle, Walking and idle variations.
I have been meaning to write something about idle variations for some time, because they aren't documented anywhere.
Idle variations are animations that must be called with the same name as idle animations, with a suffix like "_variation_0" or "_variation_1" and so on. So for example if you have these animations:
Idle_iso_nw_variation_0, Idle_iso_nw_variation_1, Idle_iso_nw_variation_2
when the character is idle and facing north-west, one of them may be played, randomly. The same animation is then played backwards some time later, so the character returns to the Idle pose.
A good example could be a character looking to one side, or crossing his arms, so it feels more like he's alive.
If you want to play any other animations manually though, it should be easy to do with SceneObject.playAnimation('animationName'). Now that I think about it though, if the IsoCharacters had a getDirection() method to know which direction they're facing, this would certainly be easier - I'll add it in the next update.